Name: Maria da Conceição Machado Gomes
Type: MSc dissertation
Publication date: 31/03/2016
Advisor:
Name |
Role |
|---|---|
| Alfredo Carlos Rodrigues Feitosa | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Alfredo Carlos Rodrigues Feitosa | Advisor * |
| Ana Paula Ferreira Nunes | Internal Examiner * |
| Luiz Alberto Sobral Vieira Junior | External Examiner * |
Summary: Dental infections and periodontal disease may act as risk factors for breast cancer. However, little is known about the composition of the periodontal microbiota in individuals with breast cancer and its relation to tooth loss and periodontal infection. OBJECTIVE: To evaluate the clinical and microbiological condition of the subgingival biofilm in women with and without breast cancer. METHODS: Case-control study and sample (n = 92) consisted of 62 women with breast cancer and 30 women without breast cancer suffering from periodontal disease. 92 samples of subgingival biofilm derived from deep periodontal sites were evaluated microbiologically. The significance level was 5% and the Mann-Whitney test (1947) was required to verify the differences between the means of case and control groups. To evaluate the correlation between the complex of bacteria in the groups, we used the Spearman
correlation. RESULTS: There is a significant association between breast cancer and loss of upper and lower teeth (p=0.002/Case; p=0.012/control), the same as bleeding on probing, but with significance for the control group (p = 0.003). With regard to the detection of bacterial species, Treponema denticola was the most prevalent species (90.0%) of red complex samples in the case group, followed by Porphyromonas gingivalis (80%), and Tannerella forsythia (70.0%). In the complex orange, Fusobacterium nucleatum species (sp. polymorphum), Fusobacterium nucleatum (sp.nucleatum) Prevotella nigrescens and were the most common (98.3%, 91.7% and 90.0%), while in the complex yellow, Streptococcus gordonii and Streptococcus oralis were the most prevalent (95.0%; 95.0%). P. gingivalis showed a positive relationship with tooth loss in the upper and lower teeth in the group with breast
cancer (p = 0.017; p = 0.038), the same is true for T. denticola (p = 0.003; p = 0.008).Bleeding on probing showed a positive and statistically significant (p = 0.014) for the upper teeth in the control group. Other bacterial species showed statistically significant associations in the case group: P. nigrescens (p = 0.028), F. nucleatum sp. polymorphum) (P = 0.002), Campylobacter showae (p <0.001), Fusobacterium periodonticum, Streptococcus intermedius (p = 0.013) and Streptococcus gordonii (p<0.001). The predominant bacterial profile for bacteria (average score of copies of DNA) in women in the control group, highlighted six microorganisms with statistical significance: T. denticola, Parvimonas micra, F. nucleatum (sp. polimorphum), F. nucleatum (sp. nucleatum) and S. oralis. CONCLUSION: Tooth loss in the presence
of the species P. gingivalis and T. denticola significantly associated with breast
cancer. In addition, women with breast cancer are 2.1 times more likely to have loss of the lower molars when there is no detection of S. intermedius species compared to the control group. As for the loss of molar teeth higher in the presence of these bacteria increase these odds by 1.2 times. Breast cancer is also associated significantly with gingival bleeding in the presence of T.denticola species.
