Molecular Microbiological Evaluation of Peri-implant and Periodontal Sites of Partially Edentulous Patients
Name: Lorrany de Barros Del Piero
Type: MSc dissertation
Publication date: 17/02/2020
Advisor:
Name |
Role |
|---|---|
| Alfredo Carlos Rodrigues Feitosa | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Alfredo Carlos Rodrigues Feitosa | Advisor * |
| Ana Paula Ferreira Nunes | Internal Examiner * |
| Stefania Carvalho Kano | Co advisor * |
Summary: Evidence related to oral microbiota from individuals treated with osseointegrated implants has been well documented in recent years, however still remains inconclusive. Objective: To evaluate the periimplantar and subgingival microbiota of patients rehabilitated with dental implants at the Dental School of the Federal University of Espírito Santo. Methodology: Cross-sectional analytical study consisted of 20 patients rehabilitated with dental implants. Probing depth, clinical attachment level, bleeding on probing and visible plaque were measured. Biofilm samples from periodontal and periimplant sites from the same patient were evaluated microbiologically using the Checkerboard DNA-DNA Hybridization technique. The significance level adopted was 5% and Qui-square, Fishers Exact, Mann-Whitney,
Kruskal-Wallis, Duncans tests and the Binomial of two proportions were required to statistical analysis. Results: Teeths visible plaque associated significantlly with 33 out 40 bacterial species. The microbiota of distal and proximal teeth was significant and prominently to 18 bacterial species. From total microbiotas teeth 21 species were significant and P. gingivalis, F. nucleatum ssp. vincentii, C. ochraceae, A. naeslundii I, N. mucosa, and S. noxia reached the highest proportion. In the microbiota of implants, the highest proportions of bacteria were for V.parvula and M.mucosa. The orange complex was the highest proportion (31.22%) found at the implants from all microbial complex, followed by distal (31.19%) and proximal teeth
(30.19%). Conclusion: The dental implantss microbiota was similar to the teeth, but in the teeth, the highest proportions of bacteria were found.
